live-cell imaging (located outside of the CNMS)
laboratory provides full capabilities to manipulate and image hydrated
Major imaging instruments
- Live Cell Zeiss LSM 710 High Resolution Confocal Microscopy
A Carl Zeiss 710 confocal microscope with an environmentally controlled stage is available for live cell multi-channel imaging of biological samples.
- Zeiss Elyra Superresolution Confocal Microscope
Structured Illumination Microscopy (SR-SIM) and Photo-activated Localization Microscopy (PAL-M) offer sub-diffraction limited resolution imaging of biological and material samples.
A Leica TCS SP2 MP scanning laser confocal laser system for multiphoton excitation and Red (HeNe, 633 nm/10mW), Green (HeNe, 543/1.2mW) and Blue (Ar 458/5mW; 476nm/5 mW; 488nm/20mW; 514nm/20mW) laser systems and a 6-channel Acousto Optical Tunable Filter for laser line selection and attenuation. The optical system is uv compatible. The inverted stage system is equipped with transmitted light detection for recording bright field images and a 50W mercury arc lamp for epifluorescent illumination. Heated sample holders and perfusion systems are in hand.
The Zeiss Axioskop 2 FS plus fluorescence microscope is equipped
with epifluorescent (top) illumination, Nomarski phase contrast
optics (bottom) and either top or bottom illumination using 12W
halogens lamps. A 12 bit Retiga color CCD camera is mounted on
the microsope for image collection. A Burleigh PCS-5000 Series
Patch Clamp Micromanipulator and ceramic objectives for electrophysiology
SPM / Fluorescence Imaging System
A Molecular Imaging PicoPlus scanning probe microscope system
is available. This system contains small (10 µm) and large
(100 µm) closed-loop multipurpose scanners with low-coherence
lasers and a Picoscan 3000 controller. The closed loop motion
control allows for reproducible positioning and lithography on
the nanometer scale. Liquid cells, flow cells and temperature
control equipment are included. The system contains magnetic
and acoustical cantilever oscillation modes and the PicoTrec
system that allows for simultaneous topography and chemical recognition.
A video imaging system allows for sample viewing from above and
through the scan head. Alternatively, the system is mountable
onto a Zeiss Axiovert 135 epifluorescent microscope.
Cell-Tracking and –Imaging
A Zeiss Axioplan 2 epifluorescent upright microscopy
system with a cooled Diagnostic Instruments Spot Xplorer 1.4
CCD camera and Ludl Electronics Products BioPrecision Stage is
available for automated image acquisition for subsequent cell
tracking analyses. At present, two fields of view are available,
namely 60 and 90µm, and they are facilitated by a long
working distance objective and oil immersion lens, respectively.
The system is computer-controlled using custom software that,
once initiated, can track, autofocus, and acquire both monochromatic
brightfield and fluorescent images repeatedly from multiple nodes
over very long times (tens of hours) without additional input
from an operator.
Synthesis of Vertically Aligned Carbon Nanofibers (VACNFs)
System with Load Lock
This system is available to grow nanofibers on substrates up
to 8 inches in diameter using DC plasma.
provided by other CNMS groups
Thursday, 31-Jan-2013 13:12:42 EST